CPSA USA 2021 – 12th November 2021

Sponsored by Mozaic Solutions

Gary Valaskovic, Mozaic Solutions – Digital LC and Precision Medicine: Sample Centric Sampling

“Building analytical workflows around the sample, rather than the analytical scientist, is the logical progression to putting the patient at the centre in a meaningful timeframe.” – Neil Spooner, Spooner Bioanalytical Solutions (CPSA Everyday / CPSA USA 2021)

Updated Forecasts. A shift toward a preference for decentralized trials and a compulsory need for the precise handling of low volume samples – once typically required for proteomics applications – has now become reality. The session led by Mozaic Solutions helped to share a common sense of purpose – to make a difference and change the standard of care via a series of coordinated deliverables – on behalf of the patient. A digital expression of existing workflows and a premier body of work led by New Objective – to lead a global rethink, reconnect, reset – featuring Sample Centric Sampling led by Spooner Bioanalytical Solutions. Everyday.

• Digital LC – A Focus on Sample Preparation
  • The analog nature of LC forces the MS to work (and wait!) on the LC timescale.
  • A digital workflow frees MS productivity.
  • Current laboratory workflows are dominated by preferences of scientists (for good reason)
  • Rethink sample preparation from patient perspective 
  • Key elements (sample) within LC-MS workflow
    • Sample Aliquot 
    • Sample Storage
    • Sample Preparation
    • Analytical Chromatography 
    • LC-ESI
    • Mass Spectrometry
• Sample Centric Sampling – Build Solutions for LC-MS and non-MS Applications
  • Patient Focus
    • Zero Dead Volume (PicoClear / IntegraFrit / PicoFrit)
    • Portable Sample Prep / LC-MS (FlowTrap / FlowChip)
    • Decentralized Trials (The Future)
    • Engage Community without Disruption of Existing Workflows (Our Reality)
    • Stepping Stone to Patient Centric Sampling (We Can Do This!)
  • High Performance LC-MS / Conventional Clinical Analysis
    • Aliquot
      • Digitize (1-50 uL)
    • In-Line SPE / PicoTrap (Zero 
    • Analytical – FlowChip / PicoChip
    • Detection – MS/MS
• Next Steps and Deliverables
  • Patient / Sample
    • Leaders: Ute Gerhard, University of Hertfordshire; Emily Ehrenfeld, Mozaic Solutions
    • Deliverables
      • Benchmarking 
      • Surveys
      • White Papers 
        • Microsampling 
  • Corporate / Custom OEM
    • Leaders: Darragh Murnane, University of Hertfordshire; Neil Spooner, Spooner Bioanalytical Solutions
    • Deliverables
      • IntegraFrit Technology
      • Custom OEM
  • Research / Model System
    • Leaders: Daniel Baker, University of Hertfordshire; Sigrid Deprez, Ghent University; Nithya Paranthaman, The Institute of Cancer Research; Gary Valaskovic, New Objective
    • Deliverables
      • Identify Model System
      • Compare Results – Familiar with New (i.e., Buspirone motif)
  • Legal / Current
    • Leaders: Christophe Stove, Ghent University; Steve Klohr, Corporate Consultant; Bill Han, Legal Consultant; Joe Siple, New Objective
    • Deliverables 
      • Goals / Hurdles
      • Present / Referenced Work
  • Operating / Steering
    • Leaders: Neil Spooner, Spooner Bioanalytical Solutions; Gary Valaskovic, New Objective; Amanda Berg, New Objective; Mike Lee Mozaic Solutions; Emily Ehrenfeld, Mozaic Solutions
    • Deliverables
      • Existing Technology / Solutions – IntegraFrit / PicoFrit / FlowChip
      • New Technology / Solutions – IntegraFrit / Segmented Flow

Lessons Learned. It is our time to do something. Together. Everyday.